Anti-Human APEX1 Polyclonal Antibody (HB917014) is a rabbit polyclonal antibody detecting APEX1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pan paniscus.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant Human APEX1 (Thr61-Leu318).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
DNA repair nuclease/redox regulator APEX1 is a ~35 kDa protein. Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are DNA repair and redox regulation of transcriptional factors. Functions as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Also incises at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules.
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6. Kim, WC. et al. (2011) Journal of molecular biology 411, 960-71. PMID: 21762700
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8. Walker, LJ. et al. (1993) Molecular and cellular biology 13, 5370-6. PMID: 8355688
9. Hirota, K. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 3633-8. PMID: 9108029
10. Ramana, CV. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 5061-6. PMID: 9560228
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-APEX1 polyclonal antibody at 1μg/ml on various samples. Lane 1: HepG2 cell lysate Lane 2: Raji cell lysate Lane 3: 293T cell lysate Lane 4: Mouse spleen lysate
