Anti-Human MAD2L2 Polyclonal Antibody (HP994014)

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概述
图片
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产品说明书

概述

货号

HP994014

品牌

abinScience

描述

Anti-Human MAD2L2 Polyclonal Antibody (HP994014) is a rabbit polyclonal antibody detecting MAD2L2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human MAD2L2 (Met1-Ser211).

靶标

Mitotic arrest deficient 2-like protein 2, Mitotic spindle assembly checkpoint protein MAD2B, MAD2L2, MAD2-like protein 2, hREV7, REV7, REV7 homolog, MAD2B

纯化方式

Purified by antigen affinity column.

Accession号

Q9UI95

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2) is a ~24 kDa protein. Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection.

1. Pfleger, CM. et al. (2001) Genes & development 15, 1759-64. PMID: 11459825

2. Chen, J. et al. (2001) Genes & development 15, 1765-70. PMID: 11459826

3. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730

4. Iwai, H. et al. (2007) Cell 130, 611-23. PMID: 17719540

5. Hong, CF. et al. (2009) The Journal of biological chemistry 284, 19613-22. PMID: 19443654

6. Gupta, R. et al. (2018) Cell 173, 972-988.e23. PMID: 29656893

7. Hara, K. et al. (2010) The Journal of biological chemistry 285, 12299-307. PMID: 20164194

8. Xie, W. et al. (2012) Protein & cell 3, 864-74. PMID: 23143872

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-MAD2L2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: 293T cell lysate

参考文献

产品说明书

Anti-Human MAD2L2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MAD2L2 Polyclonal Antibody (HP994014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MAD2L2 Polyclonal Antibody (HP994014)

概述

图片

参考文献

产品说明书

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