Anti-Human ATXN3 Polyclonal Antibody (HX010014)

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概述
图片
参考文献
产品说明书

概述

货号

HX010014

品牌

abinScience

描述

Anti-Human ATXN3 Polyclonal Antibody (HX010014) is a rabbit polyclonal antibody detecting ATXN3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human ATXN3 (Met1-Arg182).

靶标

ATX3, MJD1, MJD, SCA3, Ataxin-3, Spinocerebellar ataxia type 3 protein, ATXN3, Machado-Joseph disease protein 1

纯化方式

Purified by antigen affinity column.

Accession号

P54252

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Ataxin-3 (ATXN3) is a ~41 kDa protein. Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins. Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription. Acts as a negative regulator of mTORC1 signaling in response to amino acid deprivation by mediating deubiquitination of RHEB, thereby promoting RHEB inactivation by the TSC-TBC complex.

1. Li, F. et al. (2002) The Journal of biological chemistry 277, 45004-12. PMID: 12297501

2. Mao, Y. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 12700-5. PMID: 16118278

3. Tzvetkov, N. et al. (2007) Biological chemistry 388, 973-8. PMID: 17696782

4. Seki, T. et al. (2013) The Journal of biological chemistry 288, 17145-55. PMID: 23625928

5. Ashkenazi, A. et al. (2017) Nature 545, 108-111. PMID: 28445460

6. Yao, Y. et al. (2020) Molecular cell 80, 437-451.e6. PMID: 33157014

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-ATXN3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse liver lysate

参考文献

产品说明书

Anti-Human ATXN3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ATXN3 Polyclonal Antibody (HX010014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ATXN3 Polyclonal Antibody (HX010014)

概述

图片

参考文献

产品说明书

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