Anti-Human G6PD Polyclonal Antibody (HY124014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HY124014

品牌

abinScience

描述

Anti-Human G6PD Polyclonal Antibody (HY124014) is a rabbit polyclonal antibody detecting G6PD in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human G6PD (Met1-Leu515).

靶标

Glucose-6-phosphate 1-dehydrogenase, G6PD

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P11413

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Glucose-6-phosphate 1-dehydrogenase (G6PD) is a ~59 kDa protein. Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. Also catalyzes the conversion of NAADPH, which is produced by enzymes such as DUOX1, DUOX2 and NOX5 from NAADP and promotes Ca(2+) signaling during T cell activation, back to NAADP.

1. Gu, F. et al. (2021) Science signaling 14, eabe3800. PMID: 34784249

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-G6PD polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: LO2 cell lysate
Lane 3: A431 cell lysate
Lane 4: A549 cell lysate
Western blot

Western blot analysis was performed using anti-G6PD polyclonal antibody at 1ug/mL on mouse hippocampus.

参考文献

产品说明书

Anti-Human G6PD Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human G6PD Polyclonal Antibody (HY124014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human G6PD Polyclonal Antibody (HY124014)

概述

图片

参考文献

产品说明书

相关产品推荐

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