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Anti-Human MRPL45 Polyclonal Antibody (HP020014)

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  • 100ug
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  • 参考文献

  • 产品说明书

概述
货号HP020014
品牌abinScience
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human MRPL45 (Glu92-Ile265).
靶标Mitochondrial large ribosomal subunit protein mL45, MRPL45, 39S ribosomal protein L45, mitochondrial, L45mt, MRP-L45
纯化方式Purified by antigen affinity column.
Accession号Q9BRJ2
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Large ribosomal subunit protein mL45 (MRPL45) is a ~35 kDa protein. Component of the mitochondrial large ribosomal subunit (mt-LSU). Within the mitochondrial ribosomes, required to direct the nascent polypeptide toward the tunnel exit and position the exit at a distance from the membrane surface.

1. Brown, A. et al. (2014) Science (New York, N.Y.) 346, 718-722. PMID: 25278503
2. Amunts, A. et al. (2015) Science (New York, N.Y.) 348, 95-98. PMID: 25838379
3. Brown, A. et al. (2017) Nature structural & molecular biology 24, 866-869. PMID: 28892042
4. Itoh, Y. et al. (2021) Science (New York, N.Y.) 371, 846-849. PMID: 33602856
5. Rebelo-Guiomar, P. et al. (2022) Nature communications 13, 929. PMID: 35177605
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-MRPL45 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse heart lysate
    Lane 2: Rat brain lysate
    Lane 3: Rat heart lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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