Anti-Human GSPT1 Polyclonal Antibody (HB747014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

HB747014

abinScience

种属反应性

Human, Mouse

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human GSPT1 (Lys71-Asp499).

Eukaryotic peptide chain release factor subunit 3a, GSPT1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, G1 to S phase transition protein 1 homolog, eRF3a, ERF3A

Purified by antigen affinity column.

Accession号

P15170

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1) is a ~55 kDa protein. GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes.

1. Chauvin, C. et al. (2005) Molecular and cellular biology 25, 5801-11. PMID: 15987998

2. Cheng, Z. et al. (2009) Genes & development 23, 1106-18. PMID: 19417105

3. Hoshino, S. et al. (1989) The EMBO journal 8, 3807-14. PMID: 2511002

4. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242

5. Alkalaeva, EZ. et al. (2006) Cell 125, 1125-36. PMID: 16777602

6. Feng, T. et al. (2014) Molecular cell 53, 645-54. PMID: 24486019

7. Wang, X. et al. (2019) Cell 176, 625-635.e14. PMID: 30682371

For research use only.

产品说明书

Anti-Human GSPT1 Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

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实验相关试剂

Anti-Human GSPT1 Polyclonal Antibody (HB747014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human GSPT1 Polyclonal Antibody (HB747014)

概述

图片

参考文献

产品说明书

相关产品推荐

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