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Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody (JN089014)

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规格:
  • 50ug
  • 100ug
数量:
  • 概述

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  • 参考文献

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概述
货号JN089014
品牌abinScience
种属反应性Porphyromonas gingivalis
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Porphyromonas gingivalis kgp/prtK (Asp229-Pro683).
靶标Lys-gingipain W83, 3.4.22.47, Lysine specific cysteine protease, Lysine-specific cysteine proteinase, Porphypain, PrtK48, Lys-gingipain catalytic subunit, 39 kDa adhesin, PrtK39, 15 kDa adhesin, PrtK15, 44 kDa adhesin, PrtK44, kgp, prtK, prtP
纯化方式Purified by antigen affinity column.
Accession号Q51817
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Lys-gingipain W83 (kgp/prtK) is a ~187 kDa protein. Cysteine proteinase with a strong preference for substrates with Lys in the P1 position. Hydrolyzes bovine hemoglobin, bovine serum albumin, casein, human placental type I collagen and human IgA and IgG. Disrupts the functions of polymorphonuclear leukocytes. May act as a virulence factor in the development of peridontal disease. Involved in the coaggregation of P.gingivalis with other oral bacteria.

1. Bhogal, PS. et al. (1997) Microbiology (Reading, England) 143 ( Pt 7), 2485-2495. PMID: 9245829
2. Li, N. et al. (2011) Molecular microbiology 81, 1358-73. PMID: 21812842
3. Ganuelas, LA. et al. (2013) European journal of microbiology & immunology 3, 152-62. PMID: 24265933
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-Kgp polyclonal antibody at 1μg/ml on various samples.
    Lane 1: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 70 ug
    Lane 2: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 50 ug
    Lane 3: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 30 ug
    Lane 4: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 10 ug

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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