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Anti-Human PEX5 Polyclonal Antibody (HW731014)

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规格:
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  • 100ug
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概述
货号HW731014
品牌abinScience
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human PEX5 (His306-Gln639).
靶标Peroxisomal targeting signal 1 receptor, PTS1 receptor, PTS1-BP, PTS1R, Peroxin-5, Peroxisome receptor 1, PXR1, PEX5, Peroxisomal C-terminal targeting signal import receptor
纯化方式Purified by antigen affinity column.
Accession号P50542
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Peroxisomal targeting signal 1 receptor (PEX5/PTS1) is a ~70 kDa protein. Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle.

1. Gatto, GJ. et al. (2000) Nature structural biology 7, 1091-5. PMID: 11101887
2. Dammai, V. et al. (2001) Cell 105, 187-96. PMID: 11336669
3. Harper, CC. et al. (2003) The Journal of biological chemistry 278, 7897-901. PMID: 12456682
4. Miyata, N. et al. (2005) Molecular and cellular biology 25, 10822-32. PMID: 16314507
5. Stanley, WA. et al. (2006) Molecular cell 24, 653-663. PMID: 17157249
6. Stanley, WA. et al. (2007) BMC structural biology 7, 24. PMID: 17428317
7. Freitas, MO. et al. (2011) The Journal of biological chemistry 286, 40509-19. PMID: 21976670
8. Zhang, J. et al. (2015) Nature cell biology 17, 1259-1269. PMID: 26344566
9. Fransen, M. et al. (1995) The Journal of biological chemistry 270, 7731-6. PMID: 7706321
10. Dodt, G. et al. (1995) Nature genetics 9, 115-25. PMID: 7719337
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-PEX5 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: 293T cell lysate
    Lane 2: Mouse brain lysate
    Lane 3: Rat brain lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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