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Anti-Human MAP2K4 Polyclonal Antibody (HW751014)

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  • 50ug
  • 100ug
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概述
货号HW751014
品牌abinScience
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human MAP2K4 (Asn70-Asp399).
靶标MEK 4, MEK4, SAPK kinase 1, JNKK, c-Jun N-terminal kinase kinase 1, SEK1, JNK-activating kinase 1, MAPK/ERK kinase 4, MAPKK 4, SERK1, SKK1, SAPKK-1, SAPK/ERK kinase 1, PRKMK4, MKK4, MAP kinase kinase 4, MAP2K4, Dual specificity mitogen-activated protein kinase kinase 4, SAPKK1, Stress-activated protein kinase kinase 1, JNKK1
纯化方式Purified by antigen affinity column.
Accession号P45985
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4) is a ~44 kDa protein. Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue.

NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-MAP2K4 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: RAW264.7 cell lysate
    Lane 2: NIH3T3 cell lysate
    Lane 3: 293T cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: K562 cell lysate
    Lane 7: PC-12 cell lysate
    Lane 8: Mouse brain lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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