Anti-Human MCM3 Polyclonal Antibody (HB934014)

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50ug
100ug

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概述
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参考文献
产品说明书

HB934014

abinScience

种属反应性

Human, Mouse

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human MCM3 (Ser118-Lys656).

p102, MCM3, DNA replication licensing factor MCM3, RLF subunit beta, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3

Purified by antigen affinity column.

Accession号

P25205

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

DNA replication licensing factor MCM3 is a ~90 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division (Probable).

1. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

2. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

3. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

4. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

For research use only.

Western blot

Western blot analysis was performed using anti-MCM3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: Raji cell lysate
Lane 3: 293T cell lysate

产品说明书

Anti-Human MCM3 Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

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实验相关试剂

Anti-Human MCM3 Polyclonal Antibody (HB934014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MCM3 Polyclonal Antibody (HB934014)

概述

图片

参考文献

产品说明书

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