首页 > 产品中心 > 抗体 > 一抗

Anti-Human U2AF1 Polyclonal Antibody (HX009014)

价格:
规格:
  • 50ug
  • 100ug
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号HX009014
品牌abinScience
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human U2AF1 (Met1-Arg196).
靶标U2AFBP, U2 auxiliary factor 35 kDa subunit, U2AF1, U2 snRNP auxiliary factor small subunit, U2 small nuclear RNA auxiliary factor 1, Splicing factor U2AF 35 kDa subunit, U2AF35
纯化方式Purified by antigen affinity column.
Accession号Q01081
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Splicing factor U2AF 35 kDa subunit (U2AF1) is a ~27 kDa protein. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.

NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-U2AF1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Raji cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K562 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: Mouse kidney lysate
    Lane 6: Rat kidney lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

相关产品推荐