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Anti-Human USP11 Polyclonal Antibody (HW368014)

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概述
货号HW368014
品牌abinScience
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human USP11 (Gly77-Asn292).
靶标Ubiquitin-specific-processing protease 11, Deubiquitinating enzyme 11, UHX1, USP11, Ubiquitin carboxyl-terminal hydrolase 11, Ubiquitin thioesterase 11
纯化方式Purified by antigen affinity column.
Accession号P51784
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Ubiquitin carboxyl-terminal hydrolase 11 (USP11) is a ~109 kDa protein. Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Cleaves preferentially 'Lys-6' and 'Lys-63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys-33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'-linked ubiquitin chains (in vitro). Plays a role in the regulation of pathways leading to NF-kappa-B activation.

1. Ideguchi, H. et al. (2002) The Biochemical journal 367, 87-95. PMID: 12084015
2. Schoenfeld, AR. et al. (2004) Molecular and cellular biology 24, 7444-55. PMID: 15314155
3. Yamaguchi, T. et al. (2007) The Journal of biological chemistry 282, 33943-8. PMID: 17897950
4. Sun, W. et al. (2010) Cellular signalling 22, 386-94. PMID: 19874889
5. Wiltshire, TD. et al. (2010) The Journal of biological chemistry 285, 14565-71. PMID: 20233726
6. Harper, S. et al. (2014) Biochemistry 53, 2966-78. PMID: 24724799
7. Wang, D. et al. (2018) Journal of molecular cell biology 10, 60-73. PMID: 28992046
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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