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Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody (VK512014)

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规格:
  • 50ug
  • 100ug
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号VK512014
品牌abinScience
种属反应性Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Zaire ebolavirus NP/Nucleo(Val36-Glu351).
靶标Nucleoprotein, Nucleocapsid protein, NP
纯化方式Purified by antigen affinity column.
Accession号O72142, P18272
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Nucleoprotein (NP) is a ~83 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm.

NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-Ebola virus Nucleoprotein polyclonal antibody at 1μg/ml on various samples.
    Lane 1: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 70 ug
    Lane 2: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 50 ug
    Lane 3: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 30 ug
    Lane 4: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 10 ug

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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