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Anti-Human EHD1 Polyclonal Antibody (HP095014)

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  • 参考文献

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概述
货号HP095014
品牌abinScience
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human EHD1 (Thr20-Ser402).
靶标PAST, EHD1, PAST1, PAST homolog 1, EH domain-containing protein 1, Testilin, hPAST1
纯化方式Purified by antigen affinity column.
Accession号Q9H4M9
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

EH domain-containing protein 1 (EHD1) is a ~60 kDa protein. ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Plays a role in myoblast fusion.

1. Cai, B. et al. (2013) The Journal of biological chemistry 288, 30172-30180. PMID: 24019528
2. Naslavsky, N. et al. (2004) Molecular biology of the cell 15, 2410-22. PMID: 15020713
3. George, M. et al. (2007) BMC cell biology 8, 3. PMID: 17233914
4. Rahajeng, J. et al. (2010) The Journal of biological chemistry 285, 31918-22. PMID: 20801876
5. Xie, S. et al. (2019) Journal of cell science 132. PMID: 31615969
6. Lu, Q. et al. (2015) Nature cell biology 17, 228-240. PMID: 25686250
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-EHD1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse lung lysate
    Lane 2: Rat lung lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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