Anti-Human PELO Polyclonal Antibody (HP640014)

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规格：

50ug
100ug

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概述
图片
参考文献
产品说明书

HP640014

abinScience

种属反应性

Human, Mouse, Rat

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human PELO (Met1-Asp385).

PELO, Protein pelota homolog

Purified by antigen affinity column.

Accession号

Q9BRX2

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Protein pelota homolog is a ~43 kDa protein. Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy.

1. Pisareva, VP. et al. (2011) The EMBO journal 30, 1804-17. PMID: 21448132

2. Saito, S. et al. (2013) The Journal of biological chemistry 288, 17832-43. PMID: 23667253

3. Ikeuchi, K. et al. (2016) FEBS letters 590, 3254-63. PMID: 27543824

4. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242

5. Zinoviev, A. et al. (2020) Molecular cell 77, 1340-1349.e6. PMID: 32006463

6. Wu, Z. et al. (2018) Cell metabolism 28, 130-144.e7. PMID: 29861391

For research use only.

Western blot

Western blot analysis was performed using anti-PELO polyclonal antibody at 1μg/ml on various samples.
Lane 1: RAW264.7 cell lysate

产品说明书

Anti-Human PELO Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PELO Polyclonal Antibody (HP640014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PELO Polyclonal Antibody (HP640014)

概述

图片

参考文献

产品说明书

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