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Anti-Human WDR46 Polyclonal Antibody (HT164014)

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规格:
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概述
货号HT164014
品牌abinScience
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human WDR46 (Lys187-Glu523).
靶标WDR46, C6orf11, BING4, WD repeat-containing protein BING4, WD repeat-containing protein 46
纯化方式Purified by antigen affinity column.
Accession号O15213
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

WD repeat-containing protein 46 (WDR46) is a ~68 kDa protein. Scaffold component of the nucleolar structure. Required for localization of DDX21 and NCL to the granular compartment of the nucleolus. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.

1. Hirai, Y. et al. (2013) Genes to cells : devoted to molecular & cellular mechanisms 18, 780-97. PMID: 23848194
2. Singh, S. et al. (2021) Science (New York, N.Y.) 373, eabj5338. PMID: 34516797
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-WDR46 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Hela cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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