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Anti-Human PSMB5 Polyclonal Antibody (HB987014)

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规格:
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  • 100ug
数量:
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概述
货号HB987014
品牌abinScience
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human PSMB5 (Gly59-Pro263).
靶标MB1, PSMB5, Proteasome subunit MB1, X, Macropain epsilon chain, Proteasome subunit beta type-5, Multicatalytic endopeptidase complex epsilon chain, LMPX, Proteasome subunit X, Proteasome chain 6, Proteasome epsilon chain
纯化方式Purified by antigen affinity column.
Accession号P28074
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Proteasome subunit beta type-5 (PSMB5) is a ~28 kDa protein. Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation.

NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-PSMB5 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: LO2 cell lysate
    Lane 6: U2OS cell lysate
    Lane 7: K562 cell lysate
    Lane 8: PC-3 cell lysate
    Lane 9: MCF-7 cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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