Anti-Human LYPLA1 Polyclonal Antibody (HF852014)

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50ug
100ug

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概述
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参考文献
产品说明书

HF852014

abinScience

种属反应性

Human, Mouse, Rat

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human LYPLA1 (Met1-Asp230).

APT1, LYPLA1, LPL-I, Lysophospholipase I, LysoPLA I, LPL1, hAPT1, Acyl-protein thioesterase 1, APT-1, Lysophospholipase 1

Purified by antigen affinity column.

Accession号

O75608

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Acyl-protein thioesterase 1 (LYPLA1) is a ~24 kDa protein. Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC).

1. Hirano, T. et al. (2009) Biochimica et biophysica acta 1791, 797-805. PMID: 19439193

2. Dekker, FJ. et al. (2010) Nature chemical biology 6, 449-56. PMID: 20418879

3. Tian, L. et al. (2012) The Journal of biological chemistry 287, 14718-25. PMID: 22399288

4. Adachi, N. et al. (2016) The Journal of biological chemistry 291, 20232-46. PMID: 27481942

5. Huang, X. et al. (2023) Molecular cell 83, 3485-3501.e11. PMID: 37802024

6. Bolen, AL. et al. (2011) Journal of lipid research 52, 958-70. PMID: 21393252

For research use only.

Western blot

Western blot analysis was performed using anti-LYPLA1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse liver lysate
Lane 2: Mouse kidney lysate
Lane 3: Rat liver lysate
Lane 4: Rat kidney lysate

产品说明书

Anti-Human LYPLA1 Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human LYPLA1 Polyclonal Antibody (HF852014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human LYPLA1 Polyclonal Antibody (HF852014)

概述

图片

参考文献

产品说明书

相关产品推荐

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