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Anti-Human SEC13 Polyclonal Antibody (HX854014)

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概述
货号HX854014
品牌abinScience
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human SEC13 (Met15-Gln322).
靶标D3S1231E, SEC13L1, SEC13, SEC13-like protein 1, SEC13-related protein, Protein SEC13 homolog, SEC13R, GATOR complex protein SEC13, SEC13A
纯化方式Purified by antigen affinity column.
Accession号P55735
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Protein SEC13 homolog is a ~35 kDa protein. Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Required for the exit of adipsin (CFD/ADN), an adipocyte-secreted protein from the endoplasmic reticulum.

1. Tang, BL. et al. (1997) Molecular and cellular biology 17, 256-66. PMID: 8972206
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-SEC13 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: RAW264.7 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Hela cell lysate
    Lane 4: U87 cell lysate
    Lane 5: 293T cell lysate
    Lane 6: MCF-7 cell lysate
    Lane 7: Mouse kidney lysate
    Lane 8: Mouse liver lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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