| 货号 | HW642014 |
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| 品牌 | abinScience |
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| 种属反应性 | Human |
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| 应用 | ELISA, IHC, WB |
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| 宿主 | Rabbit |
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| 克隆类型 | Polyclonal |
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| 同种型 | IgG |
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| 免疫原 | E. coli - derived recombinant Human AKR1C3 (Met1-Tyr323). |
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| 靶标 | 3-alpha-hydroxysteroid dehydrogenase type 2, Aldo-keto reductase family 1 member C3, Testosterone 17-beta-dehydrogenase 5, 3-alpha-HSD type II, brain, DD3, DD-3, PGFS, Prostaglandin F synthase, 17-beta-HSD 5, Chlordecone reductase homolog HAKRb, Dihydrodiol dehydrogenase type I, Dihydrodiol dehydrogenase 3, HSD17B5, 17-beta-hydroxysteroid dehydrogenase type 5, HA1753, KIAA0119, DDH1, 3-alpha-HSD type 2, AKR1C3 |
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| 纯化方式 | Purified by antigen affinity column. |
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| Accession号 | P42330 |
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| 状态 | Liquid |
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| 保存溶液 |
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
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| 产品使用信息 |
| 应用方法 |
稀释比例 |
| ELISA |
1:5000-1:20000 |
| IHC |
1:50-1:500 |
| WB |
1:500-1:2000 |
|
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
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| 背景 | Aldo-keto reductase family 1 member C3 (AKR1C3) is a ~36 kDa protein. Cytosolic aldo-keto reductase that catalyzes NADPH-dependent reduction of ketosteroids to hydroxysteroids. Displays broad substrate specificity with distinct positional and stereochemistry, primarily generating 17beta-hydroxysteroids, but also 3alpha- and 20alpha-hydroxysteroids. Produces potent androgens via classical and 'backdoor'/alternative pathways. In the classical androgen metabolic pathway (biosynthesis of 5alpha-dihydrotestosterone (5alpha-DHT) via testosterone), catalyzes the reduction of delta4-androstenedione to form testosterone. In the 'backdoor' androgen metabolic pathway (biosynthesis of 5alpha-dihydrotestosterone (5alpha-DHT) via pregnanes), reduces androsterone to 5alpha-androstane-3alpha,17beta-diol preceding 5alpha-DHT secretion. 1. Penning, TM. et al. (2000) The Biochemical journal 351, 67-77. PMID: 109983482. Penning, TM. et al. (2001) Molecular and cellular endocrinology 171, 137-49. PMID: 111650223. Byrns, MC. et al. (2010) The Journal of steroid biochemistry and molecular biology 118, 177-87. PMID: 200363284. Lin, HK. et al. (1997) Molecular endocrinology (Baltimore, Md.) 11, 1971-84. PMID: 94154015. Dufort, I. et al. (1999) Endocrinology 140, 568-74. PMID: 99272797. Steckelbroeck, S. et al. (2004) The Journal of biological chemistry 279, 10784-95. PMID: 146729428. Khanna, M. et al. (1995) The Journal of biological chemistry 270, 20162-8. PMID: 76500359. Endo, S. et al. (2020) The Journal of steroid biochemistry and molecular biology 199, 105586. PMID: 31926269 |
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| Note | For research use only. |
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