Anti-Human POLB Polyclonal Antibody (HY116014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

HY116014

abinScience

种属反应性

Human, Mouse, Rat

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human POLB (Asn12-Glu335).

DNA polymerase beta, POLB

Purified by antigen affinity column.

Accession号

P06746

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

DNA polymerase beta (POLB) is a ~38 kDa protein. Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase.

1. Iwanaga, A. et al. (1999) Mutation research 435, 121-8. PMID: 10556592

2. Bennett, RA. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 7166-9. PMID: 9207062

3. Matsumoto, Y. et al. (1998) Biochemistry 37, 6456-64. PMID: 9572863

4. Lang, T. et al. (2007) Molecular and cellular biology 27, 5587-96. PMID: 17526740

5. Prasad, R. et al. (1998) The Journal of biological chemistry 273, 11121-6. PMID: 9556598

6. Prasad, R. et al. (1998) The Journal of biological chemistry 273, 15263-70. PMID: 9614142

For research use only.

产品说明书

Anti-Human POLB Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLB Polyclonal Antibody (HY116014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLB Polyclonal Antibody (HY116014)

概述

图片

参考文献

产品说明书

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