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Anti-Human GADD45GIP1 Polyclonal Antibody (HP426014)

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规格:
  • 50ug
  • 100ug
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号HP426014
品牌abinScience
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human GADD45GIP1 (Thr47-Ser222).
靶标CRIF1, GADD45GIP1, 39S ribosomal protein L59, mitochondrial, PLINP1, PLINP, MRP-L59, CKII beta-associating protein, Papillomavirus L2-interacting nuclear protein 1, PLINP-1, PRG6, CR6-interacting factor 1, MRPL59, p53-responsive gene 6 protein, Growth arrest and DNA damage-inducible proteins-interacting protein 1, Mitochondrial large ribosomal subunit protein mL64
纯化方式Purified by antigen affinity column.
Accession号Q8TAE8
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Large ribosomal subunit protein mL64 (GADD45GIP1) is a ~25 kDa protein. Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but also occur in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.

NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-GADD45GIP1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: SKOV3 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: U251 cell lysate
    Lane 4: Hela cell lysate
    Lane 5: 293T cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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