Anti-Human MEPCE Polyclonal Antibody (HP278014)

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规格：

50ug
100ug

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概述
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参考文献
产品说明书

HP278014

abinScience

种属反应性

Human, Mouse

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human MEPCE (Lys407-His689).

MEPCE, Bin3 homolog, Bicoid-interacting protein 3 homolog, 7SK snRNA methylphosphate capping enzyme, MePCE, BCDIN3

Purified by antigen affinity column.

Accession号

Q7L2J0

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

7SK snRNA methylphosphate capping enzyme (MEPCE) is a ~74 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it. Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex. MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center.

1. Jeronimo, C. et al. (2007) Molecular cell 27, 262-274. PMID: 17643375

2. Xue, Y. et al. (2010) Nucleic acids research 38, 360-9. PMID: 19906723

3. Yang, Y. et al. (2019) Nature chemical biology 15, 132-140. PMID: 30559425

4. Egloff, S. et al. (2017) The EMBO journal 36, 934-948. PMID: 28254838

5. Peng, Q. et al. (2023) Science advances 9, eadj9359. PMID: 38100593

For research use only.

产品说明书

Anti-Human MEPCE Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MEPCE Polyclonal Antibody (HP278014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MEPCE Polyclonal Antibody (HP278014)

概述

图片

参考文献

产品说明书

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