Anti-Human WEE1 Polyclonal Antibody (HB216014)

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100ug

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HB216014

abinScience

种属反应性

Human, Mouse, Rat

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human WEE1 (Asn290-Ser573).

Wee1A kinase, WEE1, Wee1-like protein kinase, WEE1hu

Purified by antigen affinity column.

Accession号

P30291

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Wee1-like protein kinase is a ~71 kDa protein. Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

1. Watanabe, N. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 4419-24. PMID: 15070733

2. Watanabe, N. et al. (1995) The EMBO journal 14, 1878-91. PMID: 7743995

3. Heald, R. et al. (1993) Cell 74, 463-74. PMID: 8348613

4. McGowan, CH. et al. (1993) The EMBO journal 12, 75-85. PMID: 8428596

For research use only.

产品说明书

Anti-Human WEE1 Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

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Anti-Human WEE1 Polyclonal Antibody (HB216014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human WEE1 Polyclonal Antibody (HB216014)

概述

图片

参考文献

产品说明书

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