Anti-RTCB Polyclonal Antibody (HV728014)

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50ug
100ug

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HV728014

abinScience

种属反应性

Human, Mouse, Rat

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human RTCB (Met1-Gly505).

C22orf28, RNA-splicing ligase RtcB homolog, RTCB, 3'-phosphate/5'-hydroxy nucleic acid ligase

Purified by antigen affinity column.

Accession号

Q9Y3I0

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

RNA-splicing ligase RTCB is a ~55 kDa protein. 3'-5' RNA ligase, catalytic subunit of the tRNA-splicing ligase complex (tRNA-LC), which is involved in the enzyme-dependent maturation of intron-containing pre-tRNAs. Functions downstream of the tRNA-splicing endonuclease that removes introns, ligating the two generated halves via phosphodiester bond formation. The ligation reaction, which requires guanosine triphosphate (GTP) and Mn(2+), proceeds through three metal-dependent steps. The first step requires ZBTB8OS/Archease and involves the guanylylation of RTCB at its active site histidine, forming a covalent GMP-histidine intermediate. Before the second step, RTCB also hydrolyzes the 2',3'-cyclic phosphate (cP) at the 3' end of the 5' tRNA exon, typically generated by the tRNA-splicing endonuclease, producing a 3' phosphate.

1. Loeff, L. et al. (2025) Nature structural & molecular biology 32, 1205-1212. PMID: 40069351

2. Popow, J. et al. (2014) Nature 511, 104-7. PMID: 24870230

3. Gerber, JL. et al. (2024) Nature communications 15, 2378. PMID: 38493148

4. Jurkin, J. et al. (2014) The EMBO journal 33, 2922-36. PMID: 25378478

5. Peschek, J. et al. (2015) EMBO reports 16, 1688-98. PMID: 26483401

For research use only.

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting RTCB (HV728014) at a concentration of 31.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of RTCB was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-RTCB polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Mouse liver lysate

产品说明书

Anti-RTCB Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

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抗体

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实验相关试剂

Anti-RTCB Polyclonal Antibody (HV728014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RTCB Polyclonal Antibody (HV728014)

概述

图片

参考文献

产品说明书

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