Anti-ALOX12B Polyclonal Antibody (HF742014)

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50ug
100ug

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概述
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参考文献
产品说明书

HF742014

abinScience

种属反应性

Human

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human ALOX12B (Leu479-Ile701).

ALOX12B, Epidermis-type lipoxygenase 12, Arachidonate 12-lipoxygenase, 12R-type, 12R-LOX, 12R-lipoxygenase

Purified by antigen affinity column.

Accession号

O75342

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Arachidonate 12-lipoxygenase, 12R-type (ALOX12B) is a ~80 kDa protein. Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.

1. Zheng, Y. et al. (2011) The Journal of biological chemistry 286, 24046-56. PMID: 21558561

2. Boeglin, WE. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 6744-9. PMID: 9618483

3. Sun, D. et al. (1998) The Journal of biological chemistry 273, 33540-7. PMID: 9837935

4. Garcia-Verdugo, I. et al. (2012) The European respiratory journal 40, 714-23. PMID: 22441738

For research use only.

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALOX12B (HF742014) at a concentration of 28 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALOX12B was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

产品说明书

Anti-ALOX12B Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

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试剂盒

实验相关试剂

Anti-ALOX12B Polyclonal Antibody (HF742014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ALOX12B Polyclonal Antibody (HF742014)

概述

图片

参考文献

产品说明书

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