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Anti-TXN Polyclonal Antibody (HY155014)

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  • 100ug
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概述
货号HY155014
品牌abinScience
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human TXN (Val2-Val105).
靶标Trx, TXN, TRX1, SASP, Hom s Trx, Thioredoxin, ATL-derived factor, ADF, TRX, Surface-associated sulphydryl protein, TRDX
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号P10599
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Thioredoxin (TXN) is a ~11 kDa protein. Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

1. Limacher, A. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 178, 389-96. PMID: 17182577
2. Glaser, AG. et al. (2008) Allergy 63, 1617-23. PMID: 19032234
3. Jacquot, JP. et al. (1990) Biochemical and biophysical research communications 173, 1375-81. PMID: 2176490
4. Mitchell, DA. et al. (2005) Nature chemical biology 1, 154-8. PMID: 16408020
5. Mitchell, DA. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 11609-14. PMID: 17606900
6. Wei, SJ. et al. (2000) Cancer research 60, 6688-95. PMID: 11118054
7. Hirota, K. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 3633-8. PMID: 9108029
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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