Anti-PARN Polyclonal Antibody (HF535014)

价格:

规格：

50ug
100ug

数量：

一键复制产品信息

概述
图片
参考文献
产品说明书

HF535014

abinScience

种属反应性

Human

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human PARN (Lys178-Asp245).

Deadenylating nuclease, Polyadenylate-specific ribonuclease, Poly(A)-specific ribonuclease PARN, Deadenylation nuclease, PARN, DAN

Purified by antigen affinity column.

Accession号

O95453

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Poly(A)-specific ribonuclease PARN is a ~73 kDa protein. 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP.

1. Gao, M. et al. (2000) Molecular cell 5, 479-88. PMID: 10882133

2. Martînez, J. et al. (2001) The Journal of biological chemistry 276, 27923-9. PMID: 11359775

3. Lai, WS. et al. (2003) Molecular and cellular biology 23, 3798-812. PMID: 12748283

4. Gherzi, R. et al. (2004) Molecular cell 14, 571-83. PMID: 15175153

5. Körner, CG. et al. (1998) The EMBO journal 17, 5427-37. PMID: 9736620

6. Berndt, H. et al. (2012) RNA (New York, N.Y.) 18, 958-72. PMID: 22442037

7. Boele, J. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 11467-72. PMID: 25049417

For research use only.

产品说明书

Anti-PARN Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PARN Polyclonal Antibody (HF535014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PARN Polyclonal Antibody (HF535014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价