Anti-XPNPEP3 Polyclonal Antibody (HV814014)

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50ug
100ug

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概述
图片
参考文献
产品说明书

HV814014

abinScience

种属反应性

Human, Mouse

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human XPNPEP3 (Glu253-Gln482).

Xaa-Pro aminopeptidase 3, APP3, XPNPEP3, Aminopeptidase P3, X-Pro aminopeptidase 3

Purified by antigen affinity column.

Accession号

Q9NQH7

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Xaa-Pro aminopeptidase 3 (XPNPEP3) is a ~57 kDa protein. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Leu-Pro-Ala. Also shows low activity towards peptides with Ala or Ser at the P1 position.

1. Inoue, M. et al. (2015) Journal of cell science 128, 656-69. PMID: 25609706

2. Singh, R. et al. (2017) The Journal of biological chemistry 292, 10035-10047. PMID: 28476889

For research use only.

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting XPNPEP3 (HV814014) at a concentration of 41.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of XPNPEP3 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-XPNPEP3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PC3 cell lysate
Lane 4: Mouse kidney lysate
Lane 5: Mouse heart lysate

产品说明书

Anti-XPNPEP3 Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-XPNPEP3 Polyclonal Antibody (HV814014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-XPNPEP3 Polyclonal Antibody (HV814014)

概述

图片

参考文献

产品说明书

相关产品推荐

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