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Anti-FLI1 Polyclonal Antibody (HX971014)

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规格:
  • 50ug
  • 100ug
数量:
  • 概述

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  • 参考文献

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概述
货号HX971014
品牌abinScience
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human FLI1 (Met1-Glu196).
靶标Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1
纯化方式Purified by antigen affinity column.
Accession号Q01543
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Friend leukemia integration 1 transcription factor (FLI1) is a ~50 kDa protein. Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

1. Stockley, J. et al. (2013) Blood 122, 4090-3. PMID: 24100448
2. Stevenson, WS. et al. (2015) Blood 126, 2027-30. PMID: 26316623
3. Saultier, P. et al. (2017) Haematologica 102, 1006-1016. PMID: 28255014
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-FLI1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: HL60 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: U937 cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: K562 cell lysate
    Lane 6: Mouse thymus lysate
    Lane 7: Mouse lung lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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