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Anti-DAAM1 Polyclonal Antibody (HV298014)

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概述
货号HV298014
品牌abinScience
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human DAAM1 (Met1-Tyr250).
靶标KIAA0666, DAAM1, Disheveled-associated activator of morphogenesis 1
纯化方式Purified by antigen affinity column.
Accession号Q9Y4D1
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Disheveled-associated activator of morphogenesis 1 (DAAM1) is a ~123 kDa protein. Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells.

1. Aspenström, P. et al. (2006) Experimental cell research 312, 2180-94. PMID: 16630611
2. Lu, J. et al. (2007) Journal of molecular biology 369, 1258-69. PMID: 17482208
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-DAAM1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Mouse liver lysate
    Lane 5: Mouse brain lysate
    Lane 6: Mouse heart lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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