Peroxidasin homolog (PXDN) is a ~165 kDa protein. Catalyzes the two-electron oxidation of bromide by hydrogen peroxide and generates hypobromite as a reactive intermediate which mediates the formation of sulfilimine cross-links between methionine and hydroxylysine residues within an uncross-linked collagen IV/COL4A1 NC1 hexamer. In turns, directly contributes to the collagen IV network-dependent fibronectin/FN and laminin assembly, which is required for full extracellular matrix (ECM)-mediated signaling. Thus, sulfilimine cross-links are essential for growth factor-induced cell proliferation and survival in endothelial cells, an event essential to basement membrane integrity. In addition, through the bromide oxidation, may promote tubulogenesis and induce angiogenesis through ERK1/2, Akt, and FAK pathways. Moreover brominates alpha2 collagen IV chain/COL4A2 at 'Tyr-1485' and leads to bromine enrichment of the basement membranes. 1. Cheng, G. et al. (2008) Free radical biology & medicine 45, 1682-94. PMID: 189296422. Péterfi, Z. et al. (2009) The American journal of pathology 175, 725-35. PMID: 195900373. Bhave, G. et al. (2012) Nature chemical biology 8, 784-90. PMID: 228429734. Lázár, E. et al. (2015) Free radical biology & medicine 83, 273-82. PMID: 257087805. Soudi, M. et al. (2015) The Journal of biological chemistry 290, 10876-90. PMID: 257130636. Colon, S. et al. (2016) The Journal of biological chemistry 291, 24009-24016. PMID: 276978417. Paumann-Page, M. et al. (2017) The Journal of biological chemistry 292, 4583-4592. PMID: 281541758. Kovács, HA. et al. (2021) Antioxidants (Basel, Switzerland) 10. PMID: 346797009. Lee, SW. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 10228-10241. PMID: 3254373410. He, C. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 15827-15836. PMID: 32571911 | 
