Anti-TOP3A Polyclonal Antibody (HC351014)

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50ug
100ug

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HC351014

abinScience

种属反应性

Human, Mouse

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human TOP3A (Lys35-Ser179).

DNA topoisomerase 3-alpha, TOP3, TOP3A, DNA topoisomerase III alpha

Purified by antigen affinity column.

Accession号

Q13472

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

DNA topoisomerase 3-alpha (TOP3A) is a ~112 kDa protein. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

1. Martin, CA. et al. (2018) American journal of human genetics 103, 221-231. PMID: 30057030

2. Nicholls, TJ. et al. (2018) Molecular cell 69, 9-23.e6. PMID: 29290614

For research use only.

产品说明书

Anti-TOP3A Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-TOP3A Polyclonal Antibody (HC351014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-TOP3A Polyclonal Antibody (HC351014)

概述

图片

参考文献

产品说明书

相关产品推荐

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