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Anti-SCARA5 Polyclonal Antibody (HV465014)

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概述
货号HV465014
品牌abinScience
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human SCARA5 (Gln118-His495).
靶标SCARA5, Scavenger receptor hlg, Scavenger receptor class A member 5
纯化方式Purified by antigen affinity column.
Accession号Q6ZMJ2
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Scavenger receptor class A member 5 (SCARA5) is a ~53 kDa protein. Ferritin receptor that mediates non-transferrin-dependent delivery of iron. Mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Preferentially binds ferritin light chain (FTL) compared to heavy chain (FTH1).

NoteFor research use only.
图片
  • Western Blot

    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SCARA5 antibody (HV465014) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (TF690314) at 0.1 μg/mL.

    Predict MW: 43 kDa
    Observed MW: 43 kDa

  • Western Blot

    Rat Muscle lysates were subjected to SDS PAGE followed by western blot with SCARA5 antibody (HV465014) at 1 μg/ml.

    Lane 1: Rat Muscle lysates

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (TF690314) at 0.1 μg/mL.

    Predict MW: 43 kDa
    Observed MW: 43 kDa

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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