Dipeptidyl peptidase 8 (DPP8) is a ~103 kDa protein. Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8. Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation. The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP8, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition. 1. Abbott, CA. et al. (2000) European journal of biochemistry 267, 6140-50. PMID: 110126663. Qi, SY. et al. (2003) The Biochemical journal 373, 179-89. PMID: 126621554. Chen, YS. et al. (2004) Protein expression and purification 35, 142-6. PMID: 150390775. Jiaang, WT. et al. (2005) Bioorganic & medicinal chemistry letters 15, 687-91. PMID: 156648386. Pitman, MR. et al. (2010) Biological chemistry 391, 959-72. PMID: 205363967. Ross, B. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1437-E1445. PMID: 293827498. Okondo, MC. et al. (2017) Nature chemical biology 13, 46-53. PMID: 278207989. Johnson, DC. et al. (2018) Nature medicine 24, 1151-1156. PMID: 2996734910. Johnson, DC. et al. (2020) Cell death & disease 11, 628. PMID: 32796818 | 
