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Anti-CTBS Polyclonal Antibody (HX869014)

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概述
货号HX869014
品牌abinScience
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human CTBS (Glu47-Arg385).
靶标CTB, Di-N-acetylchitobiase, CTBS
纯化方式Purified by antigen affinity column.
Accession号Q01459
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Di-N-acetylchitobiase (CTBS) is a ~43 kDa protein. Reducing-end exoglycosidase involved in glycan degradation. Hydrolyzes N,N'-diacetylchitobiose (GlnNAc2) and higher chitin-oligosaccharides (GlcNAc(n)) to monomeric GlcNAc acting on reducing-end residues. During N-linked glycoprotein degradation, it cleaves single GlcNAc molecules uniquely from the reducing end of N,N'-diacetylchitobiose units linking oligosaccharides to proteins, and therefore it functions after cleavage of the Asn-GlcNAc bond and asparagine removal by glycosylasparaginase.

1. Fisher, KJ. et al. (1992) The Journal of biological chemistry 267, 19607-16. PMID: 1527079
2. Park, C. et al. (2005) The Journal of biological chemistry 280, 37204-16. PMID: 16115860
3. Aronson, NN. et al. (2006) Bioscience, biotechnology, and biochemistry 70, 1537-41. PMID: 16794344
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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