Anti-CD247/CD3Z Polyclonal Antibody (HB150014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

HB150014

abinScience

种属反应性

Human

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human CD247/CD3Z (Arg52-Arg164).

CD3Z, CD247, TCRZ, T-cell receptor T3 zeta chain, T3Z, T-cell surface glycoprotein CD3 zeta chain

Purified by antigen affinity column.

Accession号

P20963

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

T-cell surface glycoprotein CD3 zeta chain (CD247/CD3Z) is a ~18 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD247/CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme.

1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049

2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158

3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

4. Iwashima, M. et al. (1994) Science (New York, N.Y.) 263, 1136-9. PMID: 7509083

For research use only.

Western blot

Western blot analysis was performed using anti-CD247 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate

产品说明书

Anti-CD247/CD3Z Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

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实验相关试剂

Anti-CD247/CD3Z Polyclonal Antibody (HB150014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CD247/CD3Z Polyclonal Antibody (HB150014)

概述

图片

参考文献

产品说明书

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