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Anti-Human IL22RA2 Polyclonal Antibody (HV100014)

价格:
规格:
  • 50ug
  • 100ug
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HV100014
品牌abinScience
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human IL22RA2 (Thr22-Pro263).
靶标IL22RA2, Cytokine receptor family type 2, soluble 1, IL-22R-alpha-2, Cytokine receptor family 2 member 10, IL-22BP, CRF2-S1, Interleukin-22-binding protein, ZcytoR16, CRF2-10, Interleukin-22 receptor subunit alpha-2, IL22BP, Cytokine receptor class-II member 10, IL-22 receptor subunit alpha-2, IL-22RA2
纯化方式Purified by antigen affinity column.
Accession号Q969J5
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Interleukin-22 receptor subunit alpha-2 (IL22RA2) is a ~30 kDa protein. Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.

NoteFor research use only.
图片
  • Immunofluorescence

    HepG2 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting IL22RA2 (HV100014) at a concentration of 29.2 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of IL22RA2 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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