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Anti-Human SRY Polyclonal Antibody (HC271014)

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规格:
  • 50ug
  • 100ug
数量:
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概述
货号HC271014
品牌abinScience
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human SRY (Met1-Leu204).
靶标TDF, Sex-determining region Y protein, Testis-determining factor, SRY
纯化方式Purified by antigen affinity column.
Accession号Q05066
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Sex-determining region Y protein (SRY) is a ~23 kDa protein. Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Involved in different aspects of gene regulation including promoter activation or repression. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'. SRY HMG box recognizes DNA by partial intercalation in the minor groove and promotes DNA bending.

1. Murphy, EC. et al. (2001) Journal of molecular biology 312, 481-99. PMID: 11563911
2. Oh, HJ. et al. (2006) Molecular and cellular endocrinology 247, 47-52. PMID: 16414182
3. Polanco, JC. et al. (2007) Developmental biology 302, 13-24. PMID: 16996051
4. Desclozeaux, M. et al. (1998) The Journal of biological chemistry 273, 7988-95. PMID: 9525897
5. Ferrari, S. et al. (1992) The EMBO journal 11, 4497-506. PMID: 1425584
6. Phillips, NB. et al. (2004) Biochemistry 43, 7066-81. PMID: 15170344
7. Giese, K. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 3368-72. PMID: 8159753
8. King, CY. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 11990-4. PMID: 8265659
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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