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Anti-GLI1 Polyclonal Antibody (HY371014)

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  • 100ug
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概述
货号HY371014
品牌abinScience
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human GLI1 (Phe2-Glu234).
靶标Oncogene GLI, GLI, Glioma-associated oncogene, GLI1, Zinc finger protein GLI1
纯化方式Purified by antigen affinity column.
Accession号P08151
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Zinc finger protein GLI1 is a ~117 kDa protein. Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling.

1. Murone, M. et al. (2000) Nature cell biology 2, 310-2. PMID: 10806483
2. Lo, HW. et al. (2009) Cancer research 69, 6790-8. PMID: 19706761
3. Maloverjan, A. et al. (2010) Experimental cell research 316, 627-37. PMID: 19878745
4. Gilder, AS. et al. (2013) Biochemical and biophysical research communications 440, 431-6. PMID: 24076122
5. Zhang, Y. et al. (2013) Nature communications 4, 2608. PMID: 24217340
6. Cherry, AL. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 2563-79. PMID: 24311597
7. Kinzler, KW. et al. (1990) Molecular and cellular biology 10, 634-42. PMID: 2105456
8. Pavletich, NP. et al. (1993) Science (New York, N.Y.) 261, 1701-7. PMID: 8378770
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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