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Anti-ARHGDIB Polyclonal Antibody (HW503014)

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  • 100ug
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  • 参考文献

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概述
货号HW503014
品牌abinScience
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human ARHGDIB (Asn66-Glu201).
靶标ARHGDIB, Rho-GDI beta, Rho GDP-dissociation inhibitor 2, RAP1GN1, Rho GDI 2, GDIA2, GDID4, Ly-GDI
纯化方式Purified by antigen affinity column.
Accession号P52566
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Rho GDP-dissociation inhibitor 2 (ARHGDIB) is a ~22 kDa protein. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Regulates reorganization of the actin cytoskeleton mediated by Rho family members.

1. Adra, CN. et al. (1993) Genes, chromosomes & cancer 8, 253-61. PMID: 7512369
2. Scherle, P. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 7568-72. PMID: 8356058
3. Leffers, H. et al. (1993) Experimental cell research 209, 165-74. PMID: 8262133
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-ARHGDIB polyclonal antibody at 1µg/ml on various samples.
    Lane 1: HL-60 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Raji cell lysate
    Lane 4: THP-1 cell lysate
    Lane 5: U937 cell lysate
    Lane 6: Mouse spleen lysate
    Lane 7: Mouse lung lysate
    Lane 8: Rat spleen lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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