Anti-Human MAGOH Polyclonal Antibody (HX135014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

HX135014

abinScience

种属反应性

Human, Mouse, Rat

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human MAGOH (Met1-Ile146).

MAGOH, Protein mago nashi homolog, MAGOHA

Purified by antigen affinity column.

Accession号

P61326

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Protein mago nashi homolog (MAGOH) is a ~17 kDa protein. Required for pre-mRNA splicing as component of the spliceosome. Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation.

1. Jurica, MS. et al. (2002) RNA (New York, N.Y.) 8, 426-39. PMID: 11991638

2. Singh, KK. et al. (2013) RNA biology 10, 1291-8. PMID: 23917022

For research use only.

Western blot

Western blot analysis was performed using anti-MAGOH polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HL60 cell lysate
Lane 3: K562 cell lysate
Lane 4: Raji cell lysate
Lane 5: Jurkat cell lysate
Lane 6: Lncap cell lysate

产品说明书

Anti-Human MAGOH Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

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实验相关试剂

Anti-Human MAGOH Polyclonal Antibody (HX135014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MAGOH Polyclonal Antibody (HX135014)

概述

图片

参考文献

产品说明书

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