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Anti-Mouse FGF basic/FGF2/bFGF Polyclonal Antibody (MY398014)

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概述
货号MY398014
品牌abinScience
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Mouse FGF2/bFGF (Ala11-Ser154).
靶标Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
纯化方式Purified by antigen affinity column.
Accession号P15655
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Fibroblast growth factor 2 (FGF2/bFGF) is a ~17 kDa protein. Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro.

NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-FGF2 polyclonal antibody at 1µg/ml on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: SKOV3 cell lysate
    Lane 3: Hela cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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