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Anti-Mouse CD276/B7-H3 Polyclonal Antibody (MV612014)

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规格:
  • 50ug
  • 100ug
数量:
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概述
货号MV612014
品牌abinScience
种属反应性Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Mouse CD276/B7-H3 (Thr26-Gly239).
经测试应用WB
靶标4Ig-B7-H3, B7 homolog 3, B7-H3, B7H3, CD276, CD276 antigen, Costimulatory molecule
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
纯化方式Purified by antigen affinity column.
Accession号Q8VE98
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

CD276 antigen (B7-H3) is a ~34 kDa protein. Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells. CD276 is the therapeutic target of omburtamab (clinical).

NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-CD276 polyclonal antibody at 1µg/ml on various samples.
    Lane 1: 293T cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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