Anti-MBD2 Polyclonal Antibody (HV150014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

HV150014

abinScience

种属反应性

Human, Mouse

ELISA, IHC, WB

Rabbit

Polyclonal

E. coli - derived recombinant Human MBD2 (Glu145-Ala411).

Methyl-CpG-binding domain protein 2, Methyl-CpG-binding protein MBD2, MBD2, DMTase, Demethylase

Purified by antigen affinity column.

Accession号

Q9UBB5

Liquid

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Methyl-CpG-binding domain protein 2 (MBD2) is a ~43 kDa protein. Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as a transcriptional repressor and plays a role in gene silencing.

1. Hendrich, B. et al. (1998) Molecular and cellular biology 18, 6538-47. PMID: 9774669

2. Tatematsu, KI. et al. (2000) Genes to cells : devoted to molecular & cellular mechanisms 5, 677-88. PMID: 10947852

3. Cramer, JM. et al. (2014) The Journal of biological chemistry 289, 1294-302. PMID: 24307175

4. Ng, HH. et al. (1999) Nature genetics 23, 58-61. PMID: 10471499

5. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440

6. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666

7. Brackertz, M. et al. (2006) Nucleic acids research 34, 397-406. PMID: 16415179

For research use only.

Western blot

Western blot analysis was performed using anti-MBD2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate

产品说明书

Anti-MBD2 Polyclonal Antibody.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MBD2 Polyclonal Antibody (HV150014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MBD2 Polyclonal Antibody (HV150014)

概述

图片

参考文献

产品说明书

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