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Anti-Human TPM4 Polyclonal Antibody (HX004014)

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规格:
  • 50ug
  • 100ug
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号HX004014
品牌abinScience
描述
Anti-Human TPM4 Polyclonal Antibody (HX004014) is a rabbit polyclonal antibody detecting TPM4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human TPM4 (Met1-Ile248).
靶标Tropomyosin alpha-4 chain, Tropomyosin-4, TM30p1, TPM4
纯化方式Purified by antigen affinity column.
Accession号P67936
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Tropomyosin alpha-4 chain (TPM4) is a ~28 kDa protein. Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.

1. Crabos, M. et al. (1991) European journal of clinical investigation 21, 472-8. PMID: 1836432
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-TPM4 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: A431 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: HT-29 cell lysate
    Lane 5: C2C12 cell lysate
    Lane 6: Jurkat cell lysate
    Lane 7: U87 cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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