Anti-Human NEIL3 Polyclonal Antibody (HA947014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HA947014

品牌

abinScience

描述

Anti-Human NEIL3 Polyclonal Antibody (HA947014) is a rabbit polyclonal antibody detecting NEIL3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human NEIL3 (Met1-Lys281).

靶标

DNA glycosylase FPG2, NEIL3, Endonuclease 8-like 3, Endonuclease VIII-like 3, Nei-like protein 3, DNA glycosylase/AP lyase Neil3

纯化方式

Purified by antigen affinity column.

Accession号

Q8TAT5

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Endonuclease 8-like 3 (NEIL3) is a ~67 kDa protein. DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases. In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity.

1. Morland, I. et al. (2002) Nucleic acids research 30, 4926-36. PMID: 12433996

2. Takao, M. et al. (2009) Genes to cells : devoted to molecular & cellular mechanisms 14, 261-70. PMID: 19170771

3. Liu, M. et al. (2012) Protein expression and purification 84, 130-9. PMID: 22569481

4. Krokeide, SZ. et al. (2013) DNA repair 12, 1159-64. PMID: 23755964

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human NEIL3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NEIL3 Polyclonal Antibody (HA947014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NEIL3 Polyclonal Antibody (HA947014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价