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Anti-Human PLK3 Polyclonal Antibody (HA090014)

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概述
货号HA090014
品牌abinScience
描述
Anti-Human PLK3 Polyclonal Antibody (HA090014) is a rabbit polyclonal antibody detecting PLK3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human PLK3 (Thr426-Ala646).
靶标FGF-inducible kinase, FNK, PLK-3, Serine/threonine-protein kinase PLK3, Cytokine-inducible serine/threonine-protein kinase, PLK3, PRK, CNK, Polo-like kinase 3, Proliferation-related kinase
纯化方式Purified by antigen affinity column.
Accession号Q9H4B4
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Serine/threonine-protein kinase PLK3 is a ~71 kDa protein. Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis.

NoteFor research use only.
图片
  • Western blot

    Lane 1: Hela cell lysate
    Lane 2: H1299 cell lysate
    Lane 3: K562 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: A549 cell lysate
    Lane 6: SKOV3 cell lysate
    Lane 7: Mouse brain lysate
    Lane 8: Mouse spleen lysate
    Lane 9: Rat brain lysate
    Lane 10: Rat heart lysate
    Lane11: Rat spleen lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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