Anti-Human RNF186 Polyclonal Antibody (HA313014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HA313014

品牌

abinScience

描述

Anti-Human RNF186 Polyclonal Antibody (HA313014) is a rabbit polyclonal antibody detecting RNF186 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human RNF186 (Gly32-Val150).

靶标

RNF186, RING finger protein 186, E3 ubiquitin-protein ligase RNF186

纯化方式

Purified by antigen affinity column.

Accession号

Q9NXI6

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

E3 ubiquitin-protein ligase RNF186 is a ~24 kDa protein. E3 ubiquitin protein ligase that is part of an apoptotic signaling pathway activated by endoplasmic reticulum stress. Stimulates the expression of proteins specific of the unfolded protein response (UPR), ubiquitinates BNIP1 and regulates its localization to the mitochondrion and induces calcium release from the endoplasmic reticulum that ultimately leads to cell apoptosis. Plays a role in the maintenance of intestinal homeostasis and clearance of enteric pathogens. Upon NOD2 stimulation, ubiquitinates the ER stress sensor activating transcription factor 6/ATF6 and promotes the unfolded protein response UPR. Participates in basal level of autophagy maintenance by regulating the ubiquitination of EPHB2 and EPHB3.

1. Wang, P. et al. (2013) Cellular signalling 25, 2320-33. PMID: 23896122

2. Ranjan, K. et al. (2021) The Journal of clinical investigation 131. PMID: 34623328

3. Zhang, H. et al. (2021) Autophagy 17, 3030-3047. PMID: 33280498

4. Lear, TB. et al. (2019) The Journal of biological chemistry 294, 16527-16534. PMID: 31586034

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human RNF186 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human RNF186 Polyclonal Antibody (HA313014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human RNF186 Polyclonal Antibody (HA313014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价