Anti-TUBB3 Polyclonal Antibody (HC498014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HC498014

品牌

abinScience

描述

Anti-TUBB3 Polyclonal Antibody (HC498014) is a rabbit polyclonal antibody detecting TUBB3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human TUBB3 (Met1-Lys450).

靶标

Tubulin beta-III, TUBB4, TUBB3, Tubulin beta-4 chain, Tubulin beta-3 chain

纯化方式

Purified by antigen affinity column.

Accession号

Q13509

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Tubulin beta-3 chain (TUBB3) is a ~50 kDa protein. Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion.

1. LaFrance, BJ. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 34996871

2. Brito, C. et al. (2024) Science (New York, N.Y.) 383, 870-876. PMID: 38305685

3. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661

4. Tischfield, MA. et al. (2010) Cell 140, 74-87. PMID: 20074521

5. Shao, Q. et al. (2017) The Journal of neuroscience : the official journal of the Society for Neuroscience 37, 5620-5633. PMID: 28483977

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-TUBB3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: N2A cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse testis lysate
Lane 4: Rat brain lysate
Lane 5: Rat testis lysate

参考文献

产品说明书

Anti-TUBB3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-TUBB3 Polyclonal Antibody (HC498014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-TUBB3 Polyclonal Antibody (HC498014)

概述

图片

参考文献

产品说明书

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